一、基本情况
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1974.12 |
1978.4 |
中国人民解放军舟山要塞区服兵役； |
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1978.4 |
1978.8 |
浙江省富阳渔山乡务农； |
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1978.9 |
1982.7 |
浙江农业大学农学系，学士； |
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1982.8 |
1985.12 |
浙江农业大学基础部，助教； |
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1986.1 |
1989.12 |
美国康奈尔大学植物系，博士生，博士； |
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1989.12 |
1991.6 |
美国华盛顿大学生物系，博士后； |
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1991.6 |
1992.6 |
北京大学生物系，博士后； |
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1992.7 |
1994.6 |
北京大学生命科学学院副教授，蛋白质工程及植物基因工程国家重点实验室副主任，学术委员会委员； |
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1994.7 |
现 在 |
北京大学生命科学学院教授，博士生导师，学术委员会委员； |
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1995.9 |
1997.3 |
美国加州伯克利大学植物基因表达中心访问教授； |
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1999.7 |
现 在 |
北京大学理学部学术委员会委员。 |
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2000.7 |
现 在 |
教育部科学技术委员会生命科学学部委员 |
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2001.7 |
现 在 |
科技部863高技术计划生物与现代农业领域专家委员会委员 |
|
2001.9 |
现 在 |
长江特聘教授 |
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2002.5 |
现 在 |
农业部国家转基因生物安全委员会委员 |
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2002.9 |
现 在 |
蛋白质工程及植物基因工程国家重点实验室主任 |
Major
Research Areas:
My laboratory is interested in two major
research areas: large-scale cloning and analyses of genes involved in early
cotton fiber development, and tuberculosis
DNA vaccine development. Using cDNA prepared from 10dpa (days post anthesis)
wild-type cotton fiber as tester and cDNA from a fuzzless-lintless (fl)
mutant as driver, we applied the RDA method to recover 280 independent cDNA
fragments related directly to early cotton fiber development. cDNA microarrays
showed that 172 genes were significantly up-regulated in elongating cotton
fibers as confirmed by in situ hybridization in representative cases.
Twenty-nine cDNAs, including a putative vacuolar (H+)-ATPase
catalytic subunit, a kinesin-like calmodulin binding protein, several
arabinogalactan proteins and key enzymes involved in long chain fatty acid
biosynthesis, accumulated to greater than 50-fold in 10 dpa fiber cells when
compared to that in 0 dpa ovules. Various upstream pathways, such as auxin
signal transduction, the MAP kinase pathway, and profilin- and expansin-induced
cell wall loosening, were also activated during the fast fiber elongation
period.
We have cloned
five genes encoding different secreted antigens Ag85B, MPT-83, MPT-64, MPT-63
and ESAT-6 from Mycobacterium Tuberculosis. We are currently developing
either combined or polyvalent DNA vaccines with different combinations.
Experimental results obtained both from mice and from cows showed that
immunization of combined DNA vaccines expressing 3 or 4 secreted antigens might
produce significant protection against microbial invasion. Our data indicated
that antigen-specific IFN-γlevels, rather than antibody levels, might be a
better criteria for protective efficacy assessment.
Besides the above two major areas, the
lab is also involved in cloning and expressional analyses of 8
large gene families (AP2-EREBP、bHLH、C3H-TYPE2(Zn)、E2F-DP、HMG-BOX、NIN-like、PCG、TCP) encoding Arabidopsis transcription factors. We have so far obtained about 250 full-length cDNAs and are
currently working on yeast expression as well as gel mobility shift assays to
study their DNA binding activities. We have done some work on elucidating
molecular mechanisms of plant apical senescence using G2 pea as a model system.
We showed that transgenic Arabidopsis plants carrying a putative chloroplast
membrane-localized calcium channel protein (the PPF1 gene) in sense
orientation flowered much later compared to control plants whereas antisense
expression of this gene rendered the recipients a very rapid life cycle. The
strength of this late-flowering phenotype correlated roughly with the level of PPF1
transcripts and with the calcium storage capacity in the chloroplasts of
different transgenic lines. We suggest that PPF1 is a novel calcium ion carrier
that regulates flowering time of higher plants by changing the Ca2+
storage capacity within chloroplasts.
三、主要发表文章（仅列出2000年以来所发表论文）
*Sheng-Jian Ji, Ying-Chun
Lu, Jian-Xun Feng, Gang Wei, Jun Li, Yong-Hui Shi, Qiang Fu, Di Liu, Jing-Chu
Luo and Yu-Xian Zhu. (2003) Isolation and Analyses of Genes Preferentially
Expressed During Early Cotton Fiber Development by Subtractive PCR and cDNA
Array. Nucleic Acids Res. 31: in
press.
*蔡宏，田霞，呼西旦，潘怡，李国利， 庄玉辉，朱玉贤。(2003) 结核分枝杆菌四价核酸疫苗免疫原性和保护效率研究。中国科学，印刷中。
苏力坦·阿巴白克力，姬生健，朱玉贤。(2003) 北非蝎昆虫毒素AaHIT1基因的原核表达和转基因研究。中国生物化学与分子生物学报，19：182-185。
潘怡，蔡宏，李淑霞，李唐，朱玉贤。(2003) MPT63核酸疫苗的制备及其免疫原性。中国生物化学与分子生物学报，19：205-209。
*Dayong Wang, Yunjian Xu, Qing Li, Xuemei
Hao, Yanhui Su, Fangzhen Sun, Yuxian Zhu. (2003) Transgenic expression of a
putative calcium transporter affects the time of arabidopsis flowering. The
Plant Journal，33: 285-292
*徐云剑，顾雪松，李珺，李晴，Peter J. Davies，朱玉贤。（2003）豌豆乙酰羟酸还原异构酶基因结构、蛋白活性分析及其调控机制研究。《中国科学》，33: 19-26
*潘怡，蔡宏，李淑霞，田霞，李唐，朱玉贤。(2003) 结核分枝杆菌组合DNA疫苗的免疫效果研究。《生物化学与生物物理学报》，35: 71-76。
苏力坦·阿巴白克力，白勇，朱玉贤。(2003) GDA2基因的克隆、原核表达与融合蛋白的纯化。《农业生物技术学报》，11: 6-10。
*Yun-Jian Xu, Da-Yong Wang,
Yu-Xian Zhu (2002) Expression of the thylakoid membrane localized PPF1
in transgenic Arabidopsis affects chloroplast development. Acta Botanica
Sinica, 41: 1314-1320。
徐云剑，苏力坦·阿巴白克力，吉栩，朱玉贤。（2002）PPF1基因C端片段在大肠杆菌中的融合表达和纯化以及抗体制备。《中国生物化学与分子生物学报》，18: 594-599。
蔡宏，潘怡，汪竟，朱玉贤。（2002）结核分枝杆菌MPT-64分泌蛋白在原核表达载体中的克隆，表达与鉴定。《中国人兽共患病杂志》，18(4):1-4
蔡宏，潘怡，汪竟，朱玉贤。（2002）结核分枝杆菌三种分泌蛋白在原核表达载体中的克隆，表达与鉴定。《中国预防兽医学报》，5（24）325-330
*蔡宏，潘怡，李国利，庄玉辉，朱玉贤，（2002）结核分枝杆菌多价核酸疫苗的构建及免疫原性。《科学通报》，47: 966-971. Cai Hong, Pan
Yi, Li Guoli, Zhuang Yuhui, Zhu Yuxian. (2002) Construction of multivalent DNA
vaccines for Mycobacterium tuberculosis and its immunogenicity. Chinese Science
Bulletin, 47(19): 1589-1593
*Sheng-Jian Ji, Feng Liu, Er-Qiu Li, Yu-Xian Zhu. (2002)
Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not
human cells. Cell research, 12(2): 143-150.
*Shengjian Ji, Yingchun Lu, Jun Li, Gang Wei, Xiujuan Liang, Yuxian
Zhu. (2002) A b-tubulin-like cDNA expressed
specically in elongating cotton fibers induces longitudinal growth of fission
yeast. Biochemical and Biophysical Research Communications. 296 (5): 1245-1250.
*Lu Yingchun, Wei Gang, Zhu Yuxian (2002) Clong whole
cellulose-synthesizing operon (ayacs Operon) from Acetobacter xylinum and
transforming it into cultivated cotton plants. Acta Botanica Sinica. 44:
441-445
*Wang Dayong, Hu Shuang, Li Qing, Cui Keming and Zhu Yuxian (2002)
Photoperiod control of the apical bud and leaf senescence in pumpkin strain
185. Acta Botanica Sinica. 44: 55-62
*苏彦辉，曲红，Vincent Vachon，张杰，罗静初，Raynald Laprade， 朱玉贤（2002）苏云金芽孢杆菌Cry1Aa蛋白а4螺旋形成跨膜离子通道的昆虫毒性试验与模型预测。《中国科学》，32: 146-152。SuYanhui, Qu Hong,
VACHON Vincent, Lu Jingchu, ZHANG Jie, LAPRADE Raynald and Zhu Yuxian
(2002) The toxicity test and hypothetical model of Bacillus thuringiensis Cry1Aa helix4. Science In China. 45: 561-567
蔡宏，朱玉贤（2001）结核杆菌核酸疫苗的研究进展。中国预防兽医学进展，4：5-9。
*徐华松，徐云剑，顾雪松，胡廷章，苏彦辉，朱玉贤（2001）豌豆乙酰羟酸还原异构酶基因的cDNA克隆、序列分析及其表达研究。科学通报，46：1022-1026。Xu Huasong, Xu Yunjian, Gu
Xuesong, Hu Tingzhang, Su Yanhui and Zhu Yuxian (2001) Cloning and analysis of
a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea. Chinese Science
Bulletin, 46: 1808-1812. IF 0.511
*Junli Zhou, Ligeng Ma, Suqiao Zhang, Yuxian Zhu, Daye Sun. (2001)
Extracellular calmodulin stimulates light-independent RbcS-GUS expression in
suspension-cultured cells of transgenic tobacco. Plant Cell Physiol, 42:
1049-1055. IF 2.30
*Guoying Jiang, Yunjian Xu, Xinsheng Zhu, Yanhui Su and Yuxian Zhu
(2001) Prokaryotically expressed Buthus martensii Karsch insect depressant
toxin has insecticidal effects. Toxicon.
39: 469-476. IF 1.366
蔡宏，呼西旦，李淑霞，张玲，李君莲，许苗，朱玉贤（2001）利用PCR技术检测537份新疆结核病人痰标本中的结核分枝杆菌。中国人蓄共患病杂志，17: 65-67
蔡宏， 李淑霞，呼西旦，李君莲，许苗，朱玉贤（2001）采用限制性酶切片段长度多样性（RFLP）和PCR指印技术对牛结核分支杆菌分裂研究。中国预防兽医学报，23：175-179
*Huang Jianqiu, Wei Zhiming, An Hailong and Zhu Yuxian (2001)
Agrobacterium tumefaciens-mediated transformation of rice with the spider
insecticidal gene conferring resistance to leaffolder and striped stem borer.
Cell Research, 11: 149-155. IF 2.00
*Yuxian Zhu, James M. Tepperman, Crag Fairchild and Peter H. Quail
(2000) Phytochrome B binds with greater apparent affinity than phychrome A to
the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domin of
PIF3. Proc. Natl. Acad. Sci.
97:13419-13424
*王学德，朱玉贤，季道蕃，蒋淑丽，李昱有（2000）棉纤维相关cDNA的克隆及其突变体中的结构变异。 科学通报，45: 1852-1857。Xuede Wang, Yuxian Zhu,
Daofan Ji, Shuli Jiang and Yuyou Li (2000) Cloning of cotton fiber-related cDNA
and its structure variants in four cotton fiber mutants. Chinese Science
Bulletin, 45: 1852-1857
苏彦辉，徐云剑，顾雪松，刘天昀，朱玉贤 (2000) 用两步PCR法克隆全长cDNA。生物化学与分子生物学学报，16: 751-754
*胡廷章，徐云剑，江国英，朱玉贤（2000）转BmKIT4基因烟草的抗虫性。植物学报，42：732－735。Tingzhang Hu, Yunjian Xu, Guoying Jiang and Yuxian Zhu (2000) Studies on insect resistance of transgenic tobacco plants carrying
the BMK IT4 gene. Acta Botanica
Sinica，42：732－735